PowerPlex® 18E System
The First 8-Color PowerPlex® STR Multiplex System for European Forensic Laboratories
- Amplifies all 12 ENFSI-recommended loci, 4 additional autosomal loci, two biological gender-determining loci (Amelogenin and DYS391) and two quality indicators within a single multiplex
- All loci—including SE33—are less than 325bp
- Optimized for use with the Spectrum CE and Spectrum Compact CE Systems
- Compatible with rapid cycling conditions for extracted DNA and database samples
Catalog Number:
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Size
Catalog Number: DC3620
Catalog Number: DG5010
Catalog Number: DM1601
Catalog Number: DG8571
Improve Your Success with Challenging Samples
The 8-color PowerPlex® 18E System allows amplification and detection of 16 autosomal loci (including 12 ENFSI recommended loci) plus Amelogenin and DYS391 for biological sex determination, making it ideal for European forensic laboratories. This multiplex system is optimized for use on the Spectrum and Spectrum Compact Systems.
- Obtain full profiles with degraded or challenging samples.
- Get results in approximately one hour.
- Quickly troubleshoot amplifications with quality indicator peaks.
An 8-Color STR Multiplex for European Forensic Laboratories
The power of 8 dye channels. This eight-color system provides co-amplification and fluorescent detection of 16 autosomal loci as well as Amelogenin and DYS391 for biological sex determination in a single multiplex kit. In addition, the PowerPlex® 18E System includes two quality indicator peaks (QIS and QIL) for at-a-glance troubleshooting of your amplification reactions.
Mini-STRs Give Better Results
Exposure to UV radiation, heat and weather can severely degrade DNA collected from crime scenes, mass disasters and missing persons cases. When amplifying these samples, larger loci will often drop out, making it difficult to obtain complete STR profiles.
All loci in the PowerPlex® 18E System are less than 325bp in size, reducing the risk of dropout and giving you more usable and informative profiles from even severely degraded DNA.
Profile of DNA extracted from an 800-year-old bone sample amplified with the PowerPlex® 18E System. When this sample is run using GlobalFiler® multiplex system, two highly discriminatory loci (SE33 and D2S1338) drop out. In the PowerPlex® 18E System, these loci are shorter, minimizing the chances of dropout and giving a profile PI (probability of inclusion) of 3.32 × 10−21.
The Smallest SE33 Locus of Any STR Multiplex
Get superior amplification for this highly discriminatory locus. The PowerPlex® 18E System includes a redesigned SE33 locus with the smallest amplicon size of any STR kit on the market.
More discriminatory power. The PowerPlex® 18E and PowerPlex® 35GY Systems have the shortest SE33 locus of any commercially available STR multiplex system.
A Sensitive System for Multiple Sample Types
The PowerPlex® 18E System reliability produces complete STR profiles from as little as 62.5pg of human DNA and usable, partial profiles from 31.25pg of human DNA. The system also handles a range of different sample types with ease, ensuring reliability when used with diverse casework samples.
Superior sensitivity. Call a high percentage of alleles even with small quantities of input DNA or large sample volumes.
2800M Control DNA–Spectrum CE System
Amplifying extracted DNA with the PowerPlex® 35GY System. The 2800M Control DNA (1ng) was amplified using the PowerPlex® 35GY System. Amplification products were mixed with CCO Internal Lane Standard 500 and separated on the Spectrum CE System. The electropherogram was generated using GeneMarker® HID Software for Spectrum CE Systems. Dye names are shown on the right.
2800M Control DNA–Spectrum Compact CE System
Amplifying Extracted DNA with the PowerPlex® 35GY System. The 2800M Control DNA (1ng) was amplified using the PowerPlex® 35GY System. Amplification products were mixed with CCO Internal Lane Standard 500 and separated on the Spectrum Compact CE System. The electropherogram was generated using GeneMarker® HID Software for Spectrum CE Systems.
Complete 8-Color Solutions for Your STR Workflow
We offer and support a complete 8-color forensic analysis workflow, including our 8-color compatible CE instruments, the Spectrum CE and Spectrum Compact CE Systems.
PowerPlex® 18E System Results—Spectrum CE Instrument
PowerPlex® 18E System Results—Spectrum Compact CE Instrument
Designed for Spectrum CE and Spectrum Compact CE Systems. PowerPlex® 18E profile of 0.5ng 2800M Control DNA amplified for 30 cycles in 25μl reactions. Electrophoresis performed on the Spectrum CE (left) and Spectrum Compact (right) CE Systems. Data analyzed using GeneMarker® HID Software for Spectrum CE Systems.
Promega forensic products are manufactured in alignment with the ISO 18385 standard. This standard ensures minimal risk of human DNA contamination for products used to collect, store and analyze biological materials for forensic purposes. Learn more.
Protocols
Complete Protocol
Specifications
Catalog Number:
What's in the box?
| Item | Part # | Size | Concentration | Available Separately |
|---|---|---|---|---|
|
2800M Control DNA |
DD710A | 1 × 25μl | 10ng/μl | View Product |
CCO Internal Lane Standard 500 |
DG857A | 1 × 250μl | ||
PowerPlex® 18E Allelic Ladder Mix |
DG860B | 1 × 100μl | ||
PowerPlex® 18E 10X Primer Pair Mix |
DK484B | 1 × 500μl | ||
PowerPlex® 18E 5X Master Mix |
DP810A | 1 × 1ml | ||
|
Water, Amplification Grade |
DW099A | 5 × 1,250μl | View Product |
SDS
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Use Restrictions
Not For Medical Diagnostic Use.Storage Conditions
U.S. Pat. No. 9,139,868, European Pat. No. 2972229, Japanese Pat. No. 6367307 and other patents pending.
U.S. Pat. Nos. 9,056,885, 10,139,400 and 10,215,751; European Pat. No. 2782916; and Japanese Pat. Nos. 6522339, 6574821 and 7227741.
What's in the box?
| Item | Part # | Size |
|---|---|---|
8C Matrix Mix |
DG854A | 1 × 150μl |
Matrix Dilution Buffer |
DM145A | 5 × 200μl |
SDS
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Use Restrictions
Not For Medical Diagnostic Use.Storage Conditions
U.S. Pat. No. 9,139,868, European Pat. No. 2972229, Japanese Pat. No. 6367307 and other patents pending.
U.S. Pat. Nos. 9,056,885, 10,139,400 and 10,215,751; European Pat. No. 2782916; and Japanese Pat. Nos. 6522339, 6574821 and 7227741.
What's in the box?
| Item | Part # | Size |
|---|---|---|
PowerPlex® 18E QI Reagent |
DM160A | 1 × 800μl |
SDS
Search for SDSCertificate of Analysis
Use Restrictions
Not For Medical Diagnostic Use.Storage Conditions
What's in the box?
| Item | Part # | Size |
|---|---|---|
CCO Internal Lane Standard 500 |
DG857A | 1 × 250μl |
SDS
Search for SDSCertificate of Analysis
Use Restrictions
Not For Medical Diagnostic Use.Storage Conditions
