5X AmpSolution™ Reagent, PunchSolution™ Kit and SwabSolution™ Kit
Rapid, Simple Preparation Methods for Swabs and Punches
- For processing of single-source reference, database and paternity samples where purification is unnecessary
- Save 2–4 hours per plate of samples
- Compatibility with most PowerPlex® Systems
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Catalog Number: DM1231
Catalog Number: DC9271
Catalog Number: DC8271
5X AmpSolution™ Reagent
The 5X AmpSolution™ Reagent streamlines the processing of swabs and punches for PowerPlex® STR analysis without compromising your results. While direct amplification from FTA® cards is transforming workflows in databasing and paternity testing labs, buccal swabs and non FTA cards are not ideal substrates for direct amplification.
Additionally, the AmpSolution™ Reagent enables direct amplification of DNA from unwashed FTA® punches, nonFTA punches and swabs from traditional PowerPlex® kits that were not originally intended for that application, minimizing the need to validate a new system to perform direct amplification.
Buccal Samples on FTA® Punches
Promega forensic products are manufactured in alignment with the ISO 18385 standard.This standard ensures minimal risk of human DNA contamination for products used to collect, store and analyze biological materials for forensic purposes. Learn more.
SwabSolution™ Kit
The SwabSolution™ Kit is used for rapid processing of swabs for STR analysis using PowerPlex® Systems. The SwabSolution™ Kit contains SwabSolution™ Reagent, which is used to generate a buccal swab extract that is added to the PowerPlex® System reaction. In addition, the SwabSolution™ Kit contains the 5X AmpSolution™ Reagent, which enables direct amplification of DNA from swabs using certain PowerPlex® Systems.
Buccal Swab Workflow
PunchSolution™ Kit
PunchSolution™ Kit works in conjunction with PowerPlex® Systems to provide rapid processing of punches from nonFTA storage cards (S&S 903, Bode Buccal Collector™ device, etc.) and robust amplification profiles. The PunchSolution™ Kit contains PunchSolution™ Reagent, which is used to pretreat nonFTA punches prior to addition of the PowerPlex® PCR amplification mix. In addition, the PunchSolution™ Kit contains the 5X AmpSolution™ Reagent, which enables direct amplification of DNA from punches.
The PunchSolution™ Kit offers a simple sample preparation process to simplify analysis. At 10μl, the volume of PunchSolution™ Reagent allows easier punch handling than other pretreatment products that only use 2μl of solution. Higher quality, easier-to-interpret profiles are obtained, compared to other methods for processing nonFTA punches.
Blood on S&S 903
One 1.2mm blood punch on S&S903 card was pretreated with PunchSolution™ Reagent as recommended in the PunchSolution™ Kit Technical Manual TMD038. Amplification was performed using the PowerPlex® 18D System. Amplified products were separated on an Applied Biosystems 3130xl Genetic Analyzer (3kV, 5-second injection).
Rapid Processing Solutions for PowerPlex® Systems
The 5X AmpSolution™ Reagent, SwabSolution™ Kit and PunchSolution™ Kit allow fast and simple processing of swabs and punches for analysis with various PowerPlex® Systems, including the PowerPlex® Fusion, 21, 18D, 16 HS, ESX, ESI, CS7 and Y23 Systems. These products are intended for preparation of single-source reference, database and paternity samples where sample purification is unnecessary.
Which Product is Right for Me?
Specifications
Catalog Number:
What's in the box?
| Item | Part # | Size | Available Separately |
|---|---|---|---|
| 5X AmpSolution™ Reagent | DM123A | 1 × 500μl | View Product |
SDS
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Use Restrictions
Not For Medical Diagnostic Use.Storage Conditions
What's in the box?
| Item | Part # | Size | Available Separately |
|---|---|---|---|
| PunchSolution™ Reagent | DM121A | 1 × 1ml | |
| 5X AmpSolution™ Reagent | DM123A | 1 × 500μl | View Product |
SDS
Search for SDSCertificate of Analysis
Use Restrictions
Not For Medical Diagnostic Use.Storage Conditions
What's in the box?
| Item | Part # | Size | Available Separately |
|---|---|---|---|
| SwabSolution™ Reagent | DM122A | 1 × 100ml | |
| 5X AmpSolution™ Reagent | DM123A | 1 × 500μl | View Product |
SDS
Search for SDSCertificate of Analysis
Use Restrictions
Not For Medical Diagnostic Use.Storage Conditions
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Related Products
Frequently Used With
PowerPlex® Fusion 6C System
6-color co-amplification and detection of 27 loci.
DC2705, DC2720, DC2780
PowerPlex® Y23 System
5-dye STR multiplex for amplification of 23 Y-STR loci.
DC2305, DC2320, DM6571
PowerPlex® 21 System
Multiplex STR system for human identification.
DC8902, DC8942
PowerPlex® ESX 17 and ESI 17 Fast Systems
Co-amplification and 5-color detection of ENFSI-recommended STR loci.
DC1710, DC1711, DC1720, DC1721, DC1731, DC1730
